Báo cáo khoa học: Allosteric properties of the GTP activated and CTP inhibited uracil phosphoribosyltransferase from the thermoacidophilic archaeon Sulfolobus solfataricus
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Theuppgene, encoding uracil phosphoribosyltransferase (UPRTase) fromthe thermoacidophilic archaeon Sulfolobus solfataricus, was cloned andexpressed inEscherichia coli. The enzyme was purified to homogeneity. Itbehaved as a tetramer in solution and showed optimal activity at pH 5.5when assayed at 60C. Enzyme activity was strongly stimulated by GTPand inhibited by CTP.
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Báo cáo khoa học: Allosteric properties of the GTP activated and CTP inhibited uracil phosphoribosyltransferase from the thermoacidophilic archaeon Sulfolobus solfataricus
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Allosteric properties of the GTP activated and CTP inhibited uracil phosphoribosyltransferase from the thermoacidophilic archaeon Sulfolobus solfataricus
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