Báo cáo khoa học: Allozyme assessment of genetic diversity within the relic Sicilian fir Abies nebrodensis (Lojac.) Mattei
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Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Allozyme assessment of genetic diversity within the relic Sicilian fir Abies nebrodensis (Lojac.) Mattei...
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Báo cáo khoa học: "Allozyme assessment of genetic diversity within the relic Sicilian fir Abies nebrodensis (Lojac.) Mattei" Original articleAllozyme assessment of genetic diversity within the relic Sicilian fir Abies nebrodensis (Lojac.) Mattei Roberta Proietti Jean-Michel Favre b Fulvio Ducci a a Sperimentale per la Selvicoltura, viale S. Margherita, 80-52100 Arezzo, Italy Istituto Laboratoire b de biologie forestière associé Inra, faculté des sciences, BP 239, 54506 Vandœuvre-lès-Nancy cedex, France (Received 9 April 1997; accepted I1 February 1999)Abstract - Allozyme markers (1 1 loci, 32 alleles) have been used to estimate the genetic diversity within the unique surviving popu-lation of the relic species Abies nebrodensis. Results were analysed in comparison with a reference system composed of 16 Italianpopulations of A. alba and one representative provenance of A. cephalonica, A. equi-trojani, A. bornmuelleriana and A. nordmanni-ana. These investigations allowed us i) to show that alleles Idh-2a and Pgi-1 a have contributed to the differentiation of the A. nebro-densis population from those of the reference system, ii) to show that the genetic diversity within A. nebrodensis is similar to that ofdynamic silver fir populations growing in analogous isolation and progressive drifting situations, while, simultaneously, a very highexcess of homozygotes is detected, iii) to identify in situ three different zones which corresponded to the diversity core of the species,one site in recolonizing phase and one site in an extinction phase. The origin of this particular situation is discussed and silviculturalinterventions to relaunch the dynamics of the species are suggested. (© Inra/Elsevier, Paris.)Abies nebrodensis / mediterranean firs / genetic diversity / allozymesRésumé - Évaluation par analyse du polymorphisme alloenzymatique, de la diversité génétique au sein de l’espèce reliqueAbies nebrodensis (Lojac.) Mattei. Des marqueurs alloenzymatiques (11 loci, 32 allèles) ont été utilisés pour évaluer la diversitégénétique au sein de la seule population existante de l’espèce relique A. nebrodensis. Les résultats, rapportés à un système deréférence composé de 16 populations italiennes d’A. alba et d’une provenance représentative d’A. cephalonica, A. equi-trojani, A.bornmuelleriana et A. nordmanianna, ont permis, (i) de montrer que la fréquence des allèles Idh-2a et Idh-2b permet de différencierA. nebrodensis des populations du système de référence, (ii) de montrer que la diversité génétique à l’intérieur d’A. nebrodensis estcomparable à celle des populations du système de référence présentant des situations d’isolement et de dérive génétique comparables,alors qu’en même temps on observe un fort excès d’homozygotes (iii) de mettre en évidence in situ trois zones différentes représen-tant respectivement, le noyau de diversité de l’espèce, un site de reconquête et un site en phase d’extinction. L’origine de cette situa-tion particulière est discutée et des mesures de gestion susceptibles de favoriser la reprise de la dynamique de l’espèce sont pro-posées. (© Inra/Elsevier, Paris.)Abies nebrodensis / sapins méditerranéens / diversité génétique / allozymes1. Introduction the Madonie range, south of the city of Cefalù on (figure 1). This species is the southernmost fir in Italy and, together with the Peloponnesus Greek fir (A. Abies nebrodensis is endemic species of Sicily [20, an Cephalonica), represents the southernmost expression of22, 26] represented by relic population of only single a the genus Abies in Europe.29 adult trees and about 20 small seedlings [30] growing* Correspondence and reprintsfavre@scbiol.u-nancy.fr recorded [30, 37]. Two clonal grafted collections includ- ing copies of 27 of the 29 compi ...
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Báo cáo khoa học: "Allozyme assessment of genetic diversity within the relic Sicilian fir Abies nebrodensis (Lojac.) Mattei" Original articleAllozyme assessment of genetic diversity within the relic Sicilian fir Abies nebrodensis (Lojac.) Mattei Roberta Proietti Jean-Michel Favre b Fulvio Ducci a a Sperimentale per la Selvicoltura, viale S. Margherita, 80-52100 Arezzo, Italy Istituto Laboratoire b de biologie forestière associé Inra, faculté des sciences, BP 239, 54506 Vandœuvre-lès-Nancy cedex, France (Received 9 April 1997; accepted I1 February 1999)Abstract - Allozyme markers (1 1 loci, 32 alleles) have been used to estimate the genetic diversity within the unique surviving popu-lation of the relic species Abies nebrodensis. Results were analysed in comparison with a reference system composed of 16 Italianpopulations of A. alba and one representative provenance of A. cephalonica, A. equi-trojani, A. bornmuelleriana and A. nordmanni-ana. These investigations allowed us i) to show that alleles Idh-2a and Pgi-1 a have contributed to the differentiation of the A. nebro-densis population from those of the reference system, ii) to show that the genetic diversity within A. nebrodensis is similar to that ofdynamic silver fir populations growing in analogous isolation and progressive drifting situations, while, simultaneously, a very highexcess of homozygotes is detected, iii) to identify in situ three different zones which corresponded to the diversity core of the species,one site in recolonizing phase and one site in an extinction phase. The origin of this particular situation is discussed and silviculturalinterventions to relaunch the dynamics of the species are suggested. (© Inra/Elsevier, Paris.)Abies nebrodensis / mediterranean firs / genetic diversity / allozymesRésumé - Évaluation par analyse du polymorphisme alloenzymatique, de la diversité génétique au sein de l’espèce reliqueAbies nebrodensis (Lojac.) Mattei. Des marqueurs alloenzymatiques (11 loci, 32 allèles) ont été utilisés pour évaluer la diversitégénétique au sein de la seule population existante de l’espèce relique A. nebrodensis. Les résultats, rapportés à un système deréférence composé de 16 populations italiennes d’A. alba et d’une provenance représentative d’A. cephalonica, A. equi-trojani, A.bornmuelleriana et A. nordmanianna, ont permis, (i) de montrer que la fréquence des allèles Idh-2a et Idh-2b permet de différencierA. nebrodensis des populations du système de référence, (ii) de montrer que la diversité génétique à l’intérieur d’A. nebrodensis estcomparable à celle des populations du système de référence présentant des situations d’isolement et de dérive génétique comparables,alors qu’en même temps on observe un fort excès d’homozygotes (iii) de mettre en évidence in situ trois zones différentes représen-tant respectivement, le noyau de diversité de l’espèce, un site de reconquête et un site en phase d’extinction. L’origine de cette situa-tion particulière est discutée et des mesures de gestion susceptibles de favoriser la reprise de la dynamique de l’espèce sont pro-posées. (© Inra/Elsevier, Paris.)Abies nebrodensis / sapins méditerranéens / diversité génétique / allozymes1. Introduction the Madonie range, south of the city of Cefalù on (figure 1). This species is the southernmost fir in Italy and, together with the Peloponnesus Greek fir (A. Abies nebrodensis is endemic species of Sicily [20, an Cephalonica), represents the southernmost expression of22, 26] represented by relic population of only single a the genus Abies in Europe.29 adult trees and about 20 small seedlings [30] growing* Correspondence and reprintsfavre@scbiol.u-nancy.fr recorded [30, 37]. Two clonal grafted collections includ- ing copies of 27 of the 29 compi ...
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