Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme
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Malate dehydrogenase (MDH) may be important in car-bohydrate and energymetabolism inmalarial parasites. ThecDNA corresponding to the MDHgene, identified onchromosome 6 of thePlasmodium falciparumgenome, wasamplified by RT-PCR, cloned and overexpressed inEscherichia coli. The recombinant PfMDH was purifiedto homogeneity and biochemically characterized as anNAD+(H)-specific MDH, which catalysed reversible inter-conversion of malate to oxaloacetate.
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Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme
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