Báo cáo khoa học: Calcium-dependent protein–protein interactions induce changes in proximity relationships of Cys48 and Cys64 in chicken skeletal troponin I
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The goal of this study was to relate conformational changesin the N-terminal domain of chicken troponin I (TnI) toCa2+activation of the actin–myosin interaction.The twocysteine residues in this region (Cys48 and Cys64) werelabeled with two sulfhydryl-reactive pyrene-containingfluorophores [N-(1-pyrene)maleimide, and N-(1-pyrene)-iodoacetamide].The labeled TnI showed a typical fluores-cence spectrum: two sharp peaks of monomer fluorescenceand a broad peak of excimer fluorescence arising from theformation of an excited dimer (excimer)....
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Báo cáo khoa học: Calcium-dependent protein–protein interactions induce changes in proximity relationships of Cys48 and Cys64 in chicken skeletal troponin I
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Báo cáo khoa học: Calcium-dependent protein–protein interactions induce changes in proximity relationships of Cys48 and Cys64 in chicken skeletal troponin I
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