Báo cáo khoa học: Characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus
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The toxicity of the pea albumin 1b (PA1b), a 37 amino-acidpeptide extracted from pea seeds, for cereal weevils (Sito-philus oryzae, Sitophilus granariusandSitophilus zeamais)was recently discovered.The mechanism of action of thisnew entomotoxin is still unknown and potentially involves atarget protein in the insect tissues.This work describes thecharacterization of a high-affinity binding site for PA1b ina microsomal fraction ofSitophilusspp.extracts.PurifiedPA1b was labeled to a high specific radioactivity(c.900 CiÆmmol)1)using125I, and the iodinated ligand wasfound to be biologically active.Binding of this ligand to themicrosomal fraction ofS. ...
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Báo cáo khoa học: Characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus
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Báo cáo khoa học: Characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus
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