Báo cáo khoa học: Characterization of Met95 mutants of a heme-regulated phosphodiesterase from Escherichia coli
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On the basis of amino acid sequences and crystal structuresof similar enzymes, it is proposed that Met95 of the heme-regulatedphosphodiesterase fromEscherichiacoli(EcDOS)acts as aheme axial ligand. Inaccordancewith this proposal,the Soret and visible optical absorption and magneticcircular dichroism spectra of the Fe(II) complexes of theMet95Ala and Met95Leu mutant proteins indicate thatthese complexes are five-coordinated high-spin, suggestingthat Met95 is an axial ligand for the Fe(II) complex. ...
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Báo cáo khoa học: Characterization of Met95 mutants of a heme-regulated phosphodiesterase from Escherichia coli
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Báo cáo khoa học: Characterization of Met95 mutants of a heme-regulated phosphodiesterase from Escherichia coli
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