Báo cáo khoa học: Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structural and functional implications
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Era is a highly conserved GTPase essential for bacterialgrowth. The N-terminal part of Era contains a conservedGTPase domain, whereas the C-terminal part of the proteincontains anRNA- andmembrane-binding domain, theKHdomain. To investigate whether the binding of Era to 16SrRNA and membrane requires its GTPase activity andwhether the GTPase domain is essential for these acti-vities, the N- and C-terminal parts of the Streptococ-cus pneumoniaeEra –Era-N(aminoacids 1–185) andEra-C(amino acids 141–299), respectively – were expressed andpurified. ...
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Báo cáo khoa học: Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structural and functional implications
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Báo cáo khoa học: Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structural and functional implications
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