Phân tích tư tưởng của nhân dân qua đoạn thơ: Những người vợ nhớ chồng… Những cuộc đời đã hóa sông núi ta trong Đất nước của Nguyễn Khoa Điềm
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Phosphoglyceratemutase (PGAM) activity inpromastigotesof theprotozoanparasiteLeishmaniamexicanais foundonlyin the cytosol. It corresponds to a cofactor-independentPGAM as it is not stimulated by 2,3-bisphosphoglycerateand is susceptible to EDTA and resistant to vanadate.We have cloned and sequenced the gene and developed aconvenient bacterial expression system and a high-yieldpurification protocol. Kinetic properties of the bacteriallyproduced protein have been determined (3-phosphoglycer-ate:Km¼0.27 ± 0.02 mM, kcat ¼434 ± 54 s)1; 2-phos-phoglycerate:Km¼0.11 ± 0.03 mM,kcat ¼199 ± 24 s)1).The activity is inhibited by phosphate but is resistant to Cl–andSO42–. ...
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Characterization of the cofactor-independent phosphoglycerate mutase from Leishmania mexicana mexicana
