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ATP-dependent Lon proteases belong to the superfamily ofAAA+proteins. Until recently, the identity of the residuesinvolved in their proteolytic active sites was not elucidated.However, the putative catalytic Ser–Lys dyad was recentlysuggested through sequence comparison of more than 100Lon proteases from various sources. The presence of thecatalytic dyadwas experimentally confirmedby site-directedmutagenesis of theEscherichia coliLon protease and bydetermination of the crystal structure of its proteolyticdomain. Furthermore, this extensive sequence analysisallowed the definition of two subfamilies of Lon proteases,LonA and LonB, based on the consensus sequences in theactive sites of their proteolytic domains. ...
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Báo cáo khoa học: Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains
