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Malassezia sympodialisis an opportunistic yeast that col-onizes human skin and may induce IgE and T cell reac-tivity in patients with atopic eczema/dermatitis syndrome(AEDS). Previously, we have cloned and expressed sixrecombinant allergens (rMala s 1 and rMala s 5 to rMal-a s 9) from this yeast. By combining high throughputscreening and phage surface display techniques, 27 com-plete and partial IgE-binding clones of M. sympodialishave been identified. Here we enlarged the panel ofrecombinant M. sympodialisallergens by RACE–PCR,cloning and nucleotide sequencing to obtain the codingsequences of two new IgE-binding clones. ...
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Cloning, expression and characterization of two new IgE-binding proteins from the yeast Malassezia sympodialis with sequence similarities to heat shock proteins and manganese superoxide dismutase
