Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli
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D-Ornithine aminomutase from Clostridium sticklandiicomprises two strongly associating subunits, OraS andOraE, with molecular masses of 12 800 and 82 900 Da.Previous studies have shown that inEscherichia colitherecombinant OraS protein is synthesized in the soluble formand OraE as inclusion bodies. Refolding experiments alsoindicate that the interactions between OraS and OraE andthe binding of either pyridoxal phosphate (PLP) or aden-osylcobalamin (AdoCbl) play important roles in therefolding process....
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Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli
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Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli
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