Phân tích tư tưởng của nhân dân qua đoạn thơ: Những người vợ nhớ chồng… Những cuộc đời đã hóa sông núi ta trong Đất nước của Nguyễn Khoa Điềm
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Saccharomyces cerevisiaeGpi3p is the UDP-GlcNAc-bind-ing andpresumed catalytic subunit of the enzyme that formsGlcNAc-phosphatidylinositol in glycosylphosphatidylinosi-tol biosynthesis. It is anessential proteinwithanEX7Emotifthat is conserved in four families of retaining glycosyl-transferases. All Gpi3ps contain a cysteine residue fourresidues C-terminal to EX7E. To test their importance forGpi3p functionin vivo,Glu289 and297 in theEX7Emotif ofS. cerevisiaeGpi3p, as well as Cys301, were altered by site-specificmutagenesis, and themutant proteins tested for theirability to complement nonviable GPI3-deleted haploids....
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Comparative importance in vivo of conserved glutamate residues in the EX7E motif retaining glycosyltransferase Gpi3p, the UDP-GlcNAc-binding subunit of the first enzyme in glycosylphosphatidylinositol assembly
