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Báo cáo khoa học: Construction of hybrid peptide synthetases for the production of a-L-aspartyl-L-phenylalanine, a precursor for the high-intensity sweetener aspartame

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Microorganisms produce a large number of pharmaco-logically and biotechnologically important peptides byusing nonribosomal peptide synthetases (NRPSs). Due totheir modular arrangement and their domain organizationNRPSs are particularly suitable for engineering recom-binant proteins for the production of novel peptides withinteresting properties. In order to compare differentstrategies of domain assembling and module fusions wefocused on the selective construction of a set of peptidesynthetases that catalyze the formation of the dipeptidea-L-aspartyl-L-phenylalanine (Asp-Phe), the precursor ofthe high-intensity sweetener a-L-aspartyl-L-phenylalaninemethyl ester (aspartame). ...
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Báo cáo khoa học: Construction of hybrid peptide synthetases for the production of a-L-aspartyl-L-phenylalanine, a precursor for the high-intensity sweetener aspartame

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