Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase
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3-Phosphoglycerate kinase (PGK) is a typical two-domain hinge-bendingenzyme with a well-structured interdomain region. The mechanism ofdomain–domain interaction and its regulation by substrate binding is notyet fully understood. Here the existence of strong cooperativity betweenthe two domains was demonstrated by following heat transitions of pigmuscle and yeast PGKs using differential scanning microcalorimetry andfluorimetry.
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase
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Report medicine traditional medicine scientific reports medicine cell proliferation oxidationTài liệu liên quan:
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