Báo cáo khoa học: Crystal structure of a family 16 endoglucanase from the hyperthermophile Pyrococcus furiosus – structural basis of substrate recognition
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Bacterial and archaeal endo-b-1,3-glucanases that belong to glycosidehydrolase family 16 share a b-jelly-roll fold, but differ significantly insequence and in substrate specificity. The crystal structure of the laminarin-ase (EC 3.2.1.39) from the hyperthermophilic archaeonPyrococcus furiosus(pfLamA) has been determined at 2.1 A˚resolution by molecular replace-ment. ThepfLamA structure reveals a kink of six residues (72–77) at theentrance of the catalytic cleft.
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Báo cáo khoa học: Crystal structure of a family 16 endoglucanase from the hyperthermophile Pyrococcus furiosus – structural basis of substrate recognition
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Crystal structure of a family 16 endoglucanase from the hyperthermophile Pyrococcus furiosus – structural basis of substrate recognition
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