Báo cáo khoa học: De novo RNA synthesis by a recombinant classical swine fever virus RNA-dependent RNA polymerase
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Classical swine fever virus nonstructural protein 5B (NS5B)encodes an RNA-dependent RNA polymerase, a keyenzyme of the viral replication complex. To better under-stand the initiation of viral RNA synthesis and to establishanin vitroreplication system, a recombinant NS5B protein,lacking the C-terminal 24-amino acid hydrophobic domain,was expressed inEscherichia coli. The truncated fusionprotein (NS5BD24) was purified on a Ni-chelating HisTrapaffinity column and demonstrated to initiate either plus-or minus-strand viral RNA synthesisde novoin a primer-independent manner but not by terminal nucleotidyletransferase activity....
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Báo cáo khoa học: De novo RNA synthesis by a recombinant classical swine fever virus RNA-dependent RNA polymerase
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Báo cáo khoa học: De novo RNA synthesis by a recombinant classical swine fever virus RNA-dependent RNA polymerase
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