Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo
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We have investigated the consequences of introducinga bisecting GlcNAc moiety into biantennary N-glycans.Computational analysis of glycan conformation with pro-longed simulation periods in vacuo and in a solvent boxrevealed two main effects: backfolding of thea1–6 arm andstacking of the bisectingGlcNAc and the neighboring Man/GlcNAc residues of both antennae.Chemoenzymatic syn-thesis produced the bisecting biantennary decasaccharideN-glycan and itsa2–3(6)-sialylated variants.
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Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo
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Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo
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