Báo cáo khoa học: Differences in substrate specificities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes
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TheCPBgenes of the protozoan parasiteLeishmania mex-icana encode stage-regulated cathepsin L-like cysteine pro-teases that are important virulence factors and are in atandem array of 19 genes. In this study, we have comparedthe substrate preferences of two CPB isoforms, CPB2.8 andCPB3, and a H84Y mutant of the latter enzyme, to analysethe roles played by the few amino acid differences betweenthe isoenzymes in determining substrate specificity. CPB3differs fromCPB2.8 at just three residues (N60D,D61NandD64S) in the mature domain. ...
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Báo cáo khoa học: Differences in substrate specificities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes
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Báo cáo khoa học: Differences in substrate specificities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes
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