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Báo cáo khoa học: Disorder–order transition of k CII promoted by low concentrations of guanidine hydrochloride suggests a stable core and a flexible C-terminus

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The CII protein of bacteriophagek, which activates thesynthesis of thekrepressor, plays a key role in the lysis–lysogeny switch. CII has a small in vivo half-life due to itsproteolytic susceptibility, and this instability is a key com-ponent for its regulatory role. The structural basis of thisinstability is not known. While studying guanidine hydro-chloride-assisted unfolding of CII, we found that lowconcentrations of the chaotrope (50–500 mM) have a con-siderable effect on the structure of this protein. This effect ismanifest in an increase in molar ellipticity, an enhancementof intrinsic tryptophanfluorescence intensityandareductionin ANS binding. ...
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Báo cáo khoa học: Disorder–order transition of k CII promoted by low concentrations of guanidine hydrochloride suggests a stable core and a flexible C-terminus

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