Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae
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accharomyces cerevisiaeis a widely used host in the pro-ductionof therapeutic peptides andproteins.Herewe reportthe identification of a novel endoprotease in S. cerevisiae.It is encoded by the CYM1gene and is specific for theC-terminus of basic residues of heterologously expressedpeptides. Gene disruption ofCYM1not only reduced theintracellular proteolysis, but also enhanced the secretion ofheterologously expressed peptides such as growth hormone,pro-B-type natriuretic peptide and pro-cholecystokinin....
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Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae
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