Báo cáo khóa học: Escherichia coli thioredoxin inhibition by cadmium Two mutually exclusive binding sites involving Cys32 and Asp26
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Observations of thioredoxin inhibition by cadmium andof a positive role for thioredoxin in protection from Cd2+led us to investigate the thioredoxin–cadmium interactionproperties.We used calorimetric and spectroscopicmethodsat different pHvalues to explore the relative contribution ofputative binding residues (Cys32, Cys35, Trp28, Trp31 andAsp26) within or near the active site. At pH 8 or 7.5two binding sites were identified by isothermal titrationcalorimetry with affinity constants of 10·106M)1and1·106M)1. ...
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Báo cáo khóa học: Escherichia coli thioredoxin inhibition by cadmium Two mutually exclusive binding sites involving Cys32 and Asp26
Nội dung trích xuất từ tài liệu:
Báo cáo khóa học: Escherichia coli thioredoxin inhibition by cadmium Two mutually exclusive binding sites involving Cys32 and Asp26
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