Báo cáo khoa học: Establishment of explants from 200-year-old Quercus petraea in culture

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Báo cáo khoa học: "Establishment of explants from 200-year-old Quercus petraea in culture" Original article Establishment of explants from 200-year-old Quercus petraea in culture S Mac An tSaoir M Kabrianis 1 Department of Agriculture for N Ireland and Department of Agricultural Botany, Queen’s University, Newforge Lane, Belfast, BT9 5PX, UK; Technological 2 Education Institute, Heraklion, Crete, GreeceSummary — As part of the ongoing EC-Cost 87 Woody Plant Research Program, methods of estab-lishing mature material of Quercus petraea (Mattuschka) Lieblein in culture were examined. Uno-pened buds taken from shoot tips were difficult to disinfect. Shoots produced from branch segmentsunder mist established well in culture but increasing branch age and decreasing branch diameter re-duced shoot production. Shoots produced from buds that had flushed inside transparent plasticbags, while still attached to the tree, established well in culture. Explants established in culture pro-duced more shoots under light containing a high proportion of the red spectrum.Quercus petraea/ tissue culture / mature / lightRésumé — Multiplication végétative de chêne sessile âgé de 200 ans. Les méthodes de multi-plication d’arbres adultes de Quercus petraea (Mattuschka) Lieblein ont été mises au point dans lecadre du groupe de recherches EC-Cost 87. Les bourgeons apicaux encore fermés sont difficiles àdésinfecter. Les pousses produites à partir de branches sous mist peuvent être mises en culturedans de bonnes conditions; cependant la production de pousses décroît avec l’âge de la branche etle diamètre des pousses. Les pousses développées à partir de bourgeons ayant débourré dans dessacs en plastique, alors qu’elles sont toujours attachées à l’arbre, se maintiennent correctement enculture. Les explants mis en culture produisent plus de branches sous une lumière ayant un spectreriche dans le rouge.Quercus petraea / culture de tissu / maturité / lumière A standard method of establishing aINTRODUCTION woody plant in culture would be to sterilize buds and place them on a suitable medi- um. Subsequent proliferation of lateralThis paper describes 3 methods used to shoots is the generally accepted methodestablish cultures from explants of mature of propagation in vitro to maintain the ge-Quercus petraea harvested from the Ros-trevor oak forest (1 of only 3 natural oak netic integrity of a clone (Jones, 1991). Other methods are compared to this basicforests left in Northern Ireland). The trees system.used in these experiments have been dat-ed by dendochronology and are between As part of the ongoing Cost 87 Woody200 and 300 years old (Pilcher, 1976). Plant Research the method of Program,Vermeer and Evers (1990) was repeated dispensed The medium into ml/container). was transparent, high-impact, polystyrene, disposa-(trunk sections of mature oaks produced ble, 170-cm containers. The cultures were 3shoots under mist and these were micro- grown at 20 °C with a 16 h photoperiod suppliedpropagated). Branches were used to de- by either Gro-Lux Thorn EMI (A) or Gelbweisstermine the thinnest section which could white Osram (B) lamps or a combination of both.be used to produce shoots, thus reducing The spectra are shown in figure 1.the potential damage to the trees. In a fur- Shoot multiplication was determined usingther effort to reduce the size of explant re- the multiplicati ...