Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase
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Although the annotation of the complete genome sequence ofMycoplasmapneumoniaedid not reveal a bacterial type I signal peptidase (SPase I) weshowed experimentally that such an activity must exist in this bacterium, bydetermining the N-terminus of the N-terminal gene product P40 of MPN142,formerly called ORF6 gene. Combining mass spectrometry with a methodfor sulfonating specifically the free amino terminal group of proteins, thecleavage site for a typical signal peptide was located between amino acids 25and 26 of the P40 precursor protein. ...
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Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase
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Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase
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