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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family

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The Mycobacterium tuberculosisgenome contains anunusually high number of proteins involved in the metabo-lism of lipids belonging to the Lip family, including variousnonlipolytic and lipolytic hydrolases. Driven by a structuralgenomic approach, we have biochemically characterized theRv1399c gene product, LipH, previously annotated as aputative lipase. Rv1399c was overexpressed inE. coliasinclusion bodies and refolded. Rv1399c efficiently hydro-lyzes soluble triacylglycerols and vinyl esters. It is inactiveagainst emulsified substrate and its catalytic activity isstrongly inhibited by the diethyl paranitrophenyl phosphate(E600). ...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family

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