Báo cáo khoa học: Fidelity of hepatitis B virus polymerase
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Although efficient vaccines are available, chronic hepatitis B(HBV) infection poses a major health problem worldwide,and prolonged treatment of chronicallyinfected HBVpatients with nucleoside analogs often results in drug-resistantHBVvariants.Therefore, it is critical toevaluate thecontribution of the HBV polymerase to mutations. FLAG-taggedwild-type (FPolE) andmutant (FPolE/D551A)HBVpolymerases have been expressed in insect cells and purified.The purified FPolE showed DNA polymerase activity,but FPolE/D551A did not, implying that the activity wasderived from FPolE....
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Báo cáo khoa học: Fidelity of hepatitis B virus polymerase
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Báo cáo khoa học: Fidelity of hepatitis B virus polymerase
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