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Báo cáo khoa học: Fluorescent and colored trinitrophenylated analogs of ATP and GTP

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Fluorescent and colored trinitrophenylated (TNP) analogsof ATP and GTP can interact with nucleotide-requiringenzymes and proteins as a substitute for the parent nucleo-tide. These analogs have strong binding affinities for mostnucleotide-requiring systems. Their bindings are easilydetected by absorption and fluorescence changes in the visi-ble region.Recent years have seendramatic developments inthe application of the TNP nucleotide analogs as spectro-scopic probes for the study on the nucleotide-interactingproperties of various enzymes and proteins including theirmutants. This review is intended as a broad overview ofcurrently extensively used applications of the nucleotideanalogs in various biological systems....
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Báo cáo khoa học: Fluorescent and colored trinitrophenylated analogs of ATP and GTP

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