Báo cáo khoa học: Fragments of pro-peptide activate mature penicillin amidase of Alcaligenes faecalis
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Penicillin amidase fromAlcaligenes faecalisis a recentlyidentifiedN-terminal nucleophilehydrolase,whichpossessesthe highest specificity constant (kcat/Km)forthehydrolysisof benzylpenicillin compared with penicillin amidases fromother sources. Similar to theEscherichia colipenicillin ami-dase, theA. faecalispenicillin amidase is maturatedin vivofrom an inactive precursor into the catalytically activeenzyme, containing one tightly bound Ca2+ion, via acomplex post-translational autocatalytic processing with amulti-step excision of a small internal pro-peptide. ...
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Báo cáo khoa học: Fragments of pro-peptide activate mature penicillin amidase of Alcaligenes faecalis
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Báo cáo khoa học: Fragments of pro-peptide activate mature penicillin amidase of Alcaligenes faecalis
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