Báo cáo khoa học: HIV-1 gp41 and gp160 are hyperthermostable proteins in a mesophilic environment
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HIV gp41(24–157) unfolds cooperatively over the pH rangeof 1.0–4.0 withTmvalues of 100C. At pH 2.8, proteinunfolding was 80% reversible and theDHvH/DHcalratio of3.7 is indicative of gp41 being trimeric. No evidence for amonomer–trimer equilibrium in the concentration range of0.3–36lMwas obtained by DSC and tryptophan fluores-cence. Glycosylation of gp41 was found to have only amarginal impact on the thermal stability. Reduction of thedisulfide bond or mutation of both cysteine residues hadonly a marginal impact on protein stability. ...
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Báo cáo khoa học: HIV-1 gp41 and gp160 are hyperthermostable proteins in a mesophilic environment
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Báo cáo khoa học: HIV-1 gp41 and gp160 are hyperthermostable proteins in a mesophilic environment
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