báo cáo khoa học: Human Papillomaviruses, 16INK4a and Akt expression in basal cell carcinoma

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Human Papillomaviruses, 16INK4a and Akt expression in basal cell carcinoma
Nội dung trích xuất từ tài liệu:
báo cáo khoa học: " Human Papillomaviruses, 16INK4a and Akt expression in basal cell carcinoma"Journal of Experimental &Clinical Cancer Research This Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. Human Papillomaviruses, 16INK4a and Akt expression in basal cell carcinomaJournal of Experimental & Clinical Cancer Research 2011, 30:108 doi:10.1186/1756-9966-30-108 Francesca Paolini (paolini@ifo.it) Angelo Carbone (gadalas@hotmail.com) Maria Benevolo (benevolo@ifo.it) Vitaliano Silipo (silipo@ifo.it) Francesca Rollo (benevolo@ifo.it) Renato Covello (covello@ifo.it) Paolo Piemonte (piemonte@ifo.it) Pasquale Frascione (frascione@ifo.it) Rodolfo Capizzi (rcapizzi@rm.unicatt.it) Caterina Catricala (catricala@ifo.it) Aldo Venuti (venuti@ifo.it) ISSN 1756-9966 Article type Research Submission date 13 October 2011 Acceptance date 14 November 2011 Publication date 14 November 2011 Article URL http://www.jeccr.com/content/30/1/108 This peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below).Articles in Journal of Experimental & Clinical Cancer Research are listed in PubMed and archived at PubMed Central. For information about publishing your research in Journal of Experimental & Clinical Cancer Research or any BioMed Central journal, go to http://www.jeccr.com/authors/instructions/ For information about other BioMed Central publications go to © 2011 Paolini et al. ; licensee BioMed Central Ltd.This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Journal of Experimental &Clinical Cancer Research http://www.biomedcentral.com/ © 2011 Paolini et al. ; licensee BioMed Central Ltd.This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Human Papillomaviruses, 16INK4a and Akt expression in basal cell carcinomaFrancesca Paolini1*, Angelo Carbone1*, Maria Benevolo2, Vitaliano Silipo3, Francesca Rollo2,Renato Covello2, Paolo Piemonte4, Pasquale Frascione4, Rodolfo Capizzi5, Caterina Catricalà3and Aldo Venuti11 Laboratory of Virology, Regina Elena National Cancer Institute, Rome, Italy.2 Department of Pathology, Regina Elena National Cancer Institute, Rome, Italy.3 Department of Dermatology-Oncology, S. Gallicano Dermatological Institute, Rome, Italy.4 SSD Dermatology, Regina Elena National Cancer Institute, Rome, Italy.5 Department of Dermatology, Catholic University of the Sacred Heart, Rome, Italy.* FP and AC equally participated as first author.Corresponding authorAldo VenutiLaboratory of Virology - Regina Elena National Cancer InstituteVia Messi d’Oro 156 – 00158 Rome ItalyPhone: +390652662521 Fax: +390652662520e-mail: venuti@ifo.itAbstractBackground The pathogenic role of beta-HPVs in non melanoma skin cancer (NMSC) ,is notstill completely understood, and literature data indicate that they might be at least cofactors inthe development of certain cutaneous squamous cell carcinomas. However, only few reportscontain data on basal cell carcinoma (BCC). The HPVs interact with many cellular proteinsaltering their function or the expression levels, like the p16INK4a and Akt. Our study aimed todetermine the presence of different beta -HPV types and the expression of p16INK4a and Akt inBCC, the commonest NMSC, in the normal appearing perilesional skin and in forehead swabof 37 immunocompetent patients. Methods the expression of p16INK4a and Akt, byimmunohistochemistry, and the HPV DNA, by nested PCR, were investigated in each sample.Results No correspondence of HPV types between BCC and swab samples was found,whereas a correspondence between perilesional skin and BCC was ascertained in the 16,7%of the patients. In BCC, 16 different types of beta HPV were found and the most frequenttypes were HPV107 (15,4%), HPV100 (11,5%) and HPV15 (11,5%) all belonging to the betaHPV species 2. Immunohistochemistry detected significant p16INK4a expression in almost alltumor samples (94,3%) with the highest percentages (>30%) of positive cells detected in 8cases. A statistically significant (p=0,012) increase of beta HPV presence was detected inp16INK4a strongly positive samples, in particular of species 2. pAkt expression was ...