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The nonreducing end of the substrate-binding site of humansalivarya-amylase contains two residues Trp58 and Trp59,which belong tob2–a2 loop of the catalytic (b/a)8barrel.While Trp59 stacks onto the substrate, the exact role ofTrp58 is unknown. To investigate its role in enzyme activitythe residue Trp58 was mutated to Ala, Leu or Tyr. Kineticanalysis of the wild-type and mutant enzymes was carriedout with starch and oligosaccharides as substrates. All threemutants exhibited a reduction in specific activity (150–180-fold lower than the wild type) with starch as substrate....
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Human salivary a-amylase Trp58 situated at subsite )2 is critical for enzyme activity
