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We have examined the status of the cytochromebc1 complex in mitochon-drial membranes from yeast mutants in which genes for one or more of thecytochromebc1 complex subunits were deleted. When membranes fromwild-type yeast were resolved by native gel electrophoresis and analyzed byimmunodecoration, the cytochrome bc1 complex was detected as a mixedpopulation of enzymes, consisting of cytochromebc1 dimers, and ternarycomplexes of cytochromebc1dimers associated with one and two copies ofthe cytochrome c oxidase complex....
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Identification and characterization of cytochrome bc1 subcomplexes in mitochondria from yeast with single and double deletions of genes encoding cytochrome bc1 subunits
