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By employing RT-PCR in conjunction with 3¢-RACE, a full-length cDNAencoding a novel zebrafish cytosolic sulfotransferase (SULT) was clonedand sequenced. Sequence analysis revealed that this zebrafish SULT (desig-nated SULT1 ST5) is, at the amino acid sequence level, close to 50%identical to human and dog SULT1B1 (thyroid hormone SULT). Arecombinant form of zebrafish SULT1 ST5 was expressed using thepGEX-2TK bacterial expression system and purified from transformedBL21 (DE3) cells.
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Identification of a novel thyroid hormone-sulfating cytosolic sulfotransferase, SULT1 ST5, from zebrafish Molecular cloning, expression, characterization and ontogenic study
