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The (ba)8-barrel is the most common enzyme fold and it is capable of cata-lyzing an enormous diversity of reactions. It follows that this scaffoldshould be an ideal starting point for engineering novel enzymes by directedevolution. However, experiments to date have utilized in vivo screens orselections and the compatibility of (ba)8-barrels with in vitro selectionmethods remains largely untested. We have investigated plasmid display asa suitablein vitro format by engineering a variant of phosphoribosylanth-ranilate isomerase (PRAI) that carried the FLAG epitope in active-site-forming loop 6. ...
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase
