Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin
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The interaction of heat shock protein with molecular mass25 kDa (HSP25) and its point mutants S77D + S81D (2Dmutant) and S15D + S77D + S81D (3D mutant) withintact and thermally denatured actinwas analyzedbymeansof fluorescence spectroscopy and ultracentrifugation. WildtypeHSP25 did not affect the polymerization of intact actin.The HSP25 3D mutant decreased the initial rate withoutaffecting the maximal extent of intact actin polymerization.G-actin heated at 40–45C was partially denatured, butretained its ability to polymerize....
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Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin
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