Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis
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The specificity of the Spodoptera frugiperda digestiveb-glycosidase (Sfbgly50) for fucosides, glucosides andgalactosides is determined by noncovalent interactions ofglycone6-OHandglycone 4-OHwith the active-site residuesQ39andE451.Site-directedmutagenesisandenzymesteady-state kinetics were described, showing that replacement ofE451 with glutamine increased the preference of Sfbgly50for glucosides in comparison to galactosides, whereasreplacing E451 with serine had the opposite effect.
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis
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