Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70
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The new antigen receptor (IgNAR) from sharks is a disul-phide bonded dimer of two protein chains,each containingone variable and five constant domains,and functions as anantibody. In order to assess the antigen-binding capabilitiesof isolated IgNAR variable domains (VNAR),we have con-structed anin vitro library incorporating synthetic CDR3regions of 15–18 residues in length. Screening of this libraryagainst the 60 kDa cytosolic domain of the 70 kDa outermembrane translocase receptor from human mitochondria(Tom70) resulted in one dominant antigen-specific clone(VNAR12F-11) after four rounds ofin vitroselection....
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70
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