Báo cáo khoa học: llozyme variation in six native oak species in Korea

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Báo cáo khoa học: "llozyme variation in six native oak species in Korea" article Original in six native oak in KoreaAllozyme variation species ZS Kim SW Lee JO Hyun 1 Department of Forest Resources, Korea University, Seoul 136-701; 2 Department of Forest Resources, Seoul National University, Suwon 440-100, South Korea variation at 6 loci was studied in 28 populations of the 6 oak species nativeSummary — Allozyme Carruth, Q aliena BI, Q dentata Thunb, Q mongolica Fisch, Q serratato Korea: Quercus acutissimaThunb, and Q variabilis BI. The proportion of polymorphic loci per population (P) averaged over the 6species was 74.6%. The average number of alleles/locus (A/L) was 2.26. The average observed andexpected heterozygosities (H He) were 0.302 and 0.298, respectively. Only a small amount (7%) of , othe observed genetic variation appeared to be interpopulational. Among the 6 species, Q serrataand Q dentata were genetically less variable than the others. Three loci could be used as markersfor distinguishing Q acutissima and Q variabilis from the other 4 species. Based on genetic identity,the 6 oaks were also clustered into 2 groups. This approach yields results similar to the current taxo-nomic treatment by morphological characteristics.allozyme / genetic variation / Quercus speciesRésumé — Variabilité allozymique chez 6 espèces de chênes indigènes de Corée. La variabili-té allozymique a été étudiée dans 28 populations appartenant à 6 espèces de chênes indigènes deCorée à partir de données issues de 6 loci :Quercus acutissima Carruth, Q aliena BI, Q dentataThunb, Q mongolica Fisch, Q serrata Thunb et Q variabilis BI. Le nombre moyen d’allèles (A/L) étaitde 2,26. Les hétérozygoties observées et théoriques (H H étaient de 0,302 et 0,298 respective- ,) oement. La variabilité entre populations ne représentait que 7% de la variabilité totale. Parmi les 6 es-pèces, Q serrata et Q dentata étaient les moins variables. Trois loci permettaient de distinguer Qacutissima et Q variabilis des 4 autres espèces. Le calcul des identités génétiques a permis de sé-parer les 6 espèces en 2 groupes.allozyme / variabilité génétique /Quercus be used to distinguish between the speciesINTRODUCTION and clarification of the systematic relation- ships among them were also points of in-Quercus acutissima Carruth, Q aliena BI, terest.Q dentata Thunb, Q mongolica Fisch,Q serrata Thunb, and Q variabilis BI arethe 6 native deciduous oaks which are dis- MATERIALS AND METHODStributed throughout Korea (Lee CB, 1987;Yim, 1991).They grow abundantly asdominant trees both in pure stands and A total of 533 mature individuals from 28 popula- tions of the 6 species were examined. From 5 tomixed with other species. This abundance 30 individuals per population were assayed. Lo-is attributed mainly to vigorous sprouting cations and the sample sizes are presented inability and viability on poor sites. In addi- table I.tion to the important role in forest ecosys- For isozyme analysis, young leaves forcedtems, they are economically valuable es- out of dormant twigs were homogenized in apecially for fuel and structural wood (Lee drop of extraction buffer (50 ml of Tris-HCl buf-CB, 1987; Yim, 1991). fer, pH 7.3, + 0.06 g of ethylenediaminetetra- acetic acid (EDTA) + 0.05 ml of merceptoetha- Due to indiscriminate exploitation of the nol + 5 g polyvinylpyrrolidone (PVP, mol wtwood and poor silvicultural treatments, 40 000)) and extracts were subjected to horizon-most oak stands in Korea exhibit very poor tal starch-gel (12.5%) electrophoresis using 2growth and quality. In regard to their eco- buffer systems. System I was that reported byno ...