Báo cáo khoa học: Mimicking phosphorylation of the small heat-shock protein aB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles
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The mammalian small heat shock proteinaB-crystallin canbe phosphorylated at three different sites, Ser19, Ser45 andSer59. We compared the intracellular distribution of wild-type, nonphosphorylatable and all possible pseudophos-phorylation mutants ofaB-crystallin by immunoblot andimmunocytochemical analyses of stable and transientlytransfected cells. We observed that pseudophosphorylationat two (especially S19D/S45D) or all three (S19D/S45D/S59D) sites induced the partial translocationofaB-crystallinfrom the detergent-soluble to the detergent-insoluble frac-tion....
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Mimicking phosphorylation of the small heat-shock protein aB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Mimicking phosphorylation of the small heat-shock protein aB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles
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