Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus
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Three NADH oxidase encoding genesnoxA-1, noxB-1andnoxCwere cloned from the genome ofArchaeoglobusfulgidus, expressed inEscherichia coli, and the gene productswere purified and characterized. Expression ofnoxA-1andnoxB-1resulted in active gene products of the expected size.ThenoxCgene was expressed as well but the protein pro-duced showed no activity in the standardNox assay. NoxA-1 and NoxB-1 are both FAD-containing enzymes withsubunit molecular masses of 48 and 69 kDa, respectively.
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Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus
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Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus
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