Báo cáo khoa học: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis
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Recent studies on type II thioesterases (TEIIs) involved inmicrobial secondary metabolism described a role for theseenzymes in the removal of short acyl-S- phosphopantetheineintermediates from misprimed holo-(acyl carrier proteins)and holo-(peptidyl carrier proteins) of polyketide synthasesand nonribosomal peptide synthetases. Because of theabsence of structural information on this class of enzymes,we performed a mutational analysis on a prototype TEIIessential for efficient productionof the lipopeptide antibioticsurfactin (TEIIsrf), which led to identificationof catalytic andstructural residues. ...
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Báo cáo khoa học: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis
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Báo cáo khoa học: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis
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