Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase
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Glycoside hydrolysis by retaining family 18 chitinasesinvolves a catalytic acid (Glu) which is part of a conservedDXDXEsequence motif that spans strand four of a (ba)8barrel (TIMbarrel) structure.Theseglycosidehydrolases areunusual in that thepositive charge emergingon theanomericcarbon after departure of the leaving group is stabilized bythe substrate itself (the N-acetyl group of the distorted )1sugar), rather than by a carboxylate group on the enzyme.
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase
Nội dung trích xuất từ tài liệu:
Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase
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