Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily
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We foundN-methyl-l-amino acid dehydrogenase activity in various bacter-ial strains, such as Pseudomonas putidaandBacillus alvei, and cloned thegene fromP. putidaATCC12633 intoEscherichia coli. The enzyme purifiedto homogeneity from recombinant E. colicatalyzed the NADPH-dependentformation of N-alkyl-l-amino acids from the corresponding a-oxo acids(e.g. pyruvate, phenylpyruvate, and hydroxypyruvate) and alkylamines (e.g.methylamine, ethylamine, and propylamine).
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily
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