Báo cáo khóa học: New insights into conformational and functional stability of human a-thrombin probed by high hydrostatic pressure
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The effects of high hydrostatic pressure (HHP) and urea onconformational transitions of humana-thrombin structurewere studiedbyfluorescence spectroscopy andbymeasuringthe catalytic activity of the enzyme. Treatment of thrombinwith urea produced a progressive red shift in the center ofmass of the intrinsic fluorescence emission spectrum, witha maximum displacement of 650 cm)1. HHP (270 MPa)shifted the centre ofmass by only 370 cm)1.HHP combinedwith a subdenaturing urea concentration (1.5M) displacedthe centre of mass by 750 cm)1....
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Báo cáo khóa học: New insights into conformational and functional stability of human a-thrombin probed by high hydrostatic pressure
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Báo cáo khóa học: New insights into conformational and functional stability of human a-thrombin probed by high hydrostatic pressure
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