Báo cáo khoa học: Oak chloroplast-DNA polymorphisms detected by restriction fragment length polymorphism (RFLP)

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Oak chloroplast-DNA polymorphisms detected by restriction fragment length polymorphism (RFLP)...
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: "Oak chloroplast-DNA polymorphisms detected by restriction fragment length polymorphism (RFLP)" Note Oak chloroplast-DNA polymorphisms detected by restriction fragment length polymorphism (RFLP) J Glössl J Schmidt 2 M Zechmeister-Machhart K Burg 1 Austrian Research Center, Department of Biotechnology, Seibersdorf; 2 Center of Applied Genetics, University of Bodenkultur, Vienna, AustriaSummary — Petunia hybrida chloroplast (cp) DNA probes were used to find restriction fragmentlength polymorphisms (RFLPs) in the cp DNA of the oak species Quercus robur and Quercus pe-traea. Five individuals have been analysed (2 Q robur and 3 Q petraea) with 18 different restrictionenzymes, and with 7 P hybrida cp DNA probes. Only 2 probes (P6 and P8) detected polymorphisms,probe P6 detected 4 polymorphisms with the restriction enzymes Aval, BglII, Clal and Xbal, whileprobe P8 detected a BglII polymorphism. fragment length polymorphism (RFLP)oak / chloroplast / restriction Polymorphisme de longueur des fragments de restriction de l’ADN chloroplas-Résumé — les chênes. Des sondes du génome chloroplastique de Petunia hybrida ont été utiliséestique chezpour la recherche de polymorphisme de longueur de fragment de restriction dans l’ADN chloroplas-tique (cp) de Quercus robur et Quercus petraea. L’ADNcp de 5 arbres (2 Q robur et 3 Q petraea) aété digéré par 18 enzymes de restriction et hybridé avec 7 sondes de P hybrida. Deux sondes seule-ment ont révélé du polymorphisme (P6 et P8) : P6 avec les enzymes de restriction Aval, BglII, ClaIet XbaI; P8 avec l’enzyme BglII. / chloroplaste / polymorphisme de longueur de fragment de restriction (RFLP)Quercus turn diminish the capacity of populationsINTRODUCTION to respond to new selective pressures. we need better insight into the Therefore,Climatic variations as well as human activ- and ecology of populations in or- geneticsities (eg environmental pollution) greatly der to minimize the negative effects of hu-influence natural ecosystems, frequently activity. manrestricting the habitat, reproductive poten- Genetic markers needed to under- aretial and number of individuals of many population’s genetic events tak- stand thespecies. ing place in forest tree species, eg, to These restrictions may reduce genetic study inheritance patterns, however, the which may in numbers of available genetic markers arevariability within populations, In our experiments, we used a Petunia hybri-very limited in forest tree species. In partic- da cpDNA library (originally described by Palmerular, little information is available on the et al, 1983), kindly provided by Dr D Neale, tooak pecies Quercus robur and Quercus identify oak cpDNA polymorphisms. DNA probespetraea, which are the focus of our inter- were labeled with [α- by random prim- P]dATP 32est, as the 2 most important oak species in ing (Boehringer-Mannheim). Filter hybridizationAustria. was performed as described by Church and Gil- bert (1984), at 50°C overnight. Three washes Direct DNA analysis by restriction frag- were made in 2 x SSC, 0.1 % (w/v) sodium dode-ment length polymorphism (RFLP), pro- c ...