Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy
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Cell-free protein synthesis offers rapidaccess toproteins thatare selectively labelledwith [15N]aminoacids andsuitable foranalysis by NMR spectroscopy without chromatographicpurification. A system based on anEscherichia colicell ex-tractwas optimizedwith regard toproteinyieldandminimalusage of15N-labelled amino acid, and examined for thepresence of metabolic by-products which could interferewith the NMR analysis. Yields of up to 1.8 mg of humancyclophilin A per mL of reactionmedium were obtained byexpression of a synthetic gene. ...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy
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