báo cáo khoa học: Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway
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báo cáo khoa học: "Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway"Xu et al. Journal of Experimental & Clinical Cancer Research 2011, 30:33http://www.jeccr.com/content/30/1/33 RESEARCH Open AccessOsthole induces G2/M arrest and apoptosis inlung cancer A549 cells by modulating PI3K/AktpathwayXiaoman Xu1, Yi Zhang2, Dan Qu1, Tingshu Jiang1 and Shengqi Li1* Abstract Background: To explore the effects of Osthole on the proliferation, cell cycle and apoptosis of human lung cancer A549 cells. Methods: Human lung cancer A549 cells were treated with Osthole at different concentrations. Cell proliferation was measured using the MTT assay. Cell cycle was evaluated using DNA flow cytometry analysis. Induction of apoptosis was determined by flow cytometry and fluorescent microscopy. The expressions of Cyclin B1, p-Cdc2, Bcl-2, Bax, t-Akt and p-Akt were evaluated by Western blotting. Results: Osthole inhibited the growth of human lung cancer A549 cells by inducing G2/M arrest and apoptosis. Western blotting demonstrated that Osthole down-regulated the expressions of Cyclin B1, p-Cdc2 and Bcl-2 and up-regulated the expressions of Bax in A549 cells. Inhibition of PI3K/Akt signaling pathway was also observed after treating A549 cells with Osthole. Conclusions: Our findings suggest that Osthole may have a therapeutic application in the treatment of human lung cancer.Background Recent studies have revealed that Osthole may have antiproliferative [7], vasorelaxant [8], anti-inflammatoryLung cancer is the leading cause of cancer-related death [9], antimicrobacterial [10], antiallergic [11], and pre-in the world, and non-small cell lung cancer accounts for venting prophylactic effects in hepatitis [12]. Further-approximately 80% of all cases [1,2]. Despite advances in more, the anticancer effect of Osthole has been reporteddiagnostic and therapeutic, the overall 5-year survival in few papers [13-17]. These studies have revealed thatrate in many countries is generally less than 15% [3]. In Osthole inhibited the growth, invasion and metastasis oforder to improve the survival rate, intensive efforts have cancer cells. However, the effects of Osthole on humanbeen made to find new anticancer agents, and many lung cancer cells remain unclear.attentions have been drawn to herbal medicines owing to The PI3K/Akt signaling pathway is a critical transduc-their wide range of biological activities, low toxicity and tion pathway which plays an important role in regulatingside effects [4-6]. cell proliferation, cell cycle and apoptosis [18]. Various Osthole, 7-methoxy-8-(3-methyl-2-butenyl)coumarin types of cancer, including lung cancer, were reported to(Figure 1), is an active constituent of Cnidium monnieri aberrantly activate this pathway [19]. Recent studies have(L.) Cusson, has been extracted from many medicinal shown that some anticancer-drugs could induce G2/Mplants such as Cnidium monnieri and other plants. Ost- arrest accompanying the down-regulation of Akt [20,21].hole has long been used in traditional Chinese medicine And the PI3K/Akt pathway participates in the regulationfor the treatment of eczema, cutaneous pruritus, tricho- of Bcl-2 family proteins, which are key regulators of themonas vaginalis infection, and sexual dysfunction. apoptotic pathway [22]. In the present study, we observed that Osthole induces G2/M arrest and apoptosis in lung* Correspondence: shengqi-li@hotmail.com1 Department of Respiratory Medicine, the Shengjing Hospital, China Medical cancer A549 cells. Osthole-induced G2/M arrest andUniversity, Shenyang 110004, PR China apoptosis were associated with inhibition of the CyclinFull list of author information is available at the end of the article © 2011 Xu et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Com ...