Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool
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In Klebsiella pneumoniae, the flavoprotein, NifL regulatesNifA mediated transcriptional activation of the N2-fixation(nif) genes in response tomolecular O2and ammonium.Weinvestigated the influence of membrane-bound oxidoreduc-tases on nif-regulation by biochemical analysis of purifiedNifLandbymonitoringNifA-mediated expressionofnifH¢-¢lacZ reporter fusions in different mutant backgrounds.NifL-bound FAD-cofactor was reduced by NADH only inthe presence of a redox-mediator or inside-out vesiclesderived from anaerobically grown K.pneumoniaecells,...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool
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