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Root cultures ofSwertia chirata(Gentianaceae) were grownwith supplements of [1-13C]glucose, [U-13C6]glucose or[carboxy-13C]shikimic acid. 1,3,5,8-Tetrahydroxyxanthonewas isolated and analysed by quantitative NMR analysis.The observed isotopomer distribution shows that 1,3,5,8-tetrahydroxyxanthone is biosynthesized via a polyketide-type pathway. The starter unit, 3-hydroxybenzoyl-CoA, isobtained from an early shikimate pathway intermediate.Phenylalanine, cinnamic acid and benzoic acid were ruledout as intermediates....
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Phenylalanine-independent biosynthesis of 1,3,5,8-tetrahydroxyxanthone A retrobiosynthetic NMR study with root cultures of Swertia chirata
