Báo cáo khoa học: Polyphenolic and enzymatic characterization of ageing and rejuvenation of hybrid walnut trees (Juglans nigra x Juglans regia): relationship to growth

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Báo cáo khoa học: "Polyphenolic and enzymatic characterization of ageing and rejuvenation of hybrid walnut trees (Juglans nigra x Juglans regia): relationship to growth"Polyphenolic and enzymatic characterizationof ageing and rejuvenation of hybrid walnut trees(Juglans nigra x Juglans regia): relationship to growth A. Drouet 2 D. CornuC. 1 A. Ouaras Jay-Allemand1 Station dAm6lioration des Arbres Forestiers, INRA, Ardon 45160 Olh and et, l2 Laboratoire de Physiologie du Vieillissement et de la S6nescence de.s V6gdtaux Sup6rieurs, Uni-versit6 d’Ort6ans, BP 6749, 45067 Orleans Cedex 02, France Key results and a brief discussion onIntroduction different factors involved in walnut ageing and rejuvenation will be presented.This paper is mainly devoted to the poly-phenolic and enzymatic characterizationof hybrid walnut rejuvenation in order tofind better plant materials, treatments andconditions which are propitious to propa- Materials and Methodsgation. Consequently, comparative study aof adult and rejuvenated annual shoots Two clones of x J. hybrid walnut (Juglans nigra undertaken.was regia), which grown in nursery in were a Rejuvenation, widely linked to vegeta- Orleans, were used. Each clone was repre-tive propagation, was described by Bonga sented by 2 different physiological situations: 1) a rejuvenated 13 yr old tree obtained by(1982) and it has been shown on hybrid annual coppicing in March for 10 yr, each stumpwalnut that the propagation success by in producing at least 60 sprouts; 2) 10 yr old adultvitro culture of cuttings was strongly form obtained from a cutting of a previousdependent upon severe annual coppicing stump sprout.(Cornu, 1977). First results with phenolic A detailed study of growth was undertaken tocompounds and enzymes were obtained that samples were shoots at the same ensureduring the annual growth of walnut: growth stage and to study the relationship be- tween growth rate and biochemical factors. For1) juvenility and rejuvenation seemed to each sample, 5 shoots were cut, defoliated andbe linked to high values of the ratio of typi- immediately immersed in liquid nitrogen for sub-cal polyphenols during the first stages of were taken on sequent lyophilization. Samplesgrowth after bud burst (Jay-Allemand et 5 shoot elongation (10, 18 and 24 days during June; 2 July and 7 August) (Drouet et al.,al., 1987; 1988); 2) enzyme activity 1989). Extraction, purification and high perfor-changes were found between adult and mance liquid chromatography (HPLC) methodsrejuvenated shoots, while no difference were reported by Jay-Allemand et al. (1988)was noted in the total protein content and enzyme analyses were described by(Drouet et al., 1989). Drouet et al. (1989). first growth wave, during the other hand, marked by 2 PAL peaks rejuvenation was which were absent in adult shoots (Fig. 2). However, no simple relationsh ...