Báo cáo khoa học: Preventing nondesired RNA-primed RNA extension catalyzed by T7 RNA polymerase
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The transcription patterns of 64 linear double strandedDNA templates obtained with T7 RNA polymerase wereinvestigated. These templates consisted of 17 nucleotide-long sequences under the control of the minimal bac-teriophage T7 promoter and represented all possiblecombinations of nucleotides at positions +8, +10 and+11. Two clearly distinct types of template were identified,which produced the range of transcription patternsobserved: (a) those that yielded 17-nucleotide-long RNA asthe only detectable run-off product (only 15% of the total),and (b) templates that in addition to the expected full-lengthRNA,...
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Báo cáo khoa học: Preventing nondesired RNA-primed RNA extension catalyzed by T7 RNA polymerase
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Báo cáo khoa học: Preventing nondesired RNA-primed RNA extension catalyzed by T7 RNA polymerase
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